Protein_Domain

Part:BBa_K112307:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-21)


{< lambda holin!} in BBb format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc


Source

pSB3C6-Bjh1502

References